Abstract: Purpose: To depict the anatomy variation of the cystic arteries and the biliary system, and evaluate the feasibility of Laparoscope Cholecystectomy (LC) and the relevance between the images and the operation with preoperative 64-row detectors multiple slices CT (64-MSCT). Materials and Methods: A total of 22 consecutive patients (12 males, 10 females), who subsequently underwent LC, were examined from February 2009 to May 2009. The appearances of their MSCT were compared with the results that were seen in the surgical or LC operation. Results: The cystic arteries were delineated in 20 of the 22 patients. One cystic artery was seen in the Calot triangle in 19 patients, and one cystic artery was seen out of the Calot’t triangle in 1 patient. One cystic artery was not seen in 1 patient and one cystic artery above which Hilar cavernous hemangioma was seen next to it was very tiny in 1 patient of the other two. There were 18 patients with cystic duct directly into the common bile duct by its right side, 1 patient with cystic duct into the common bile duct by its left side, 1 patient with the tortuous cystic duct and 1 patient with cystic duct into the common bile duct in the posterior segment of duodenum. There were 17 patients who were diagnosed as cholecystolithiasis, 5 patients who were diagnosed as cholecystitis, which were confirmed in the operation, 1 patient who was diagnosed as the tumor in the duodenum which press the common bile, and was underwent the open surgery in which the diagnosis was confirmed and 1 patient who was diagnosed as hilar cavernous hemangioma, and was underwent the open surgery. Conclusion:Preoperative 64-MSCT can depict the anatomy of cystic artery and biliary system and find the anatomic variation and assess the feasibility of LC as well as the difficulty degree.