Abstract: The rigid polyurethane foam with high mechanical properties, as structure materials, can be obtained by filling reinforcing materials. Structure characteristic and material composition influence mechanical properties of RRPUF. In this thesis, 3D structure of glass fiber RRPUF was tested by microfocus cone-beam X-ray industrial computed tomography. Structure characteristic such as shape, position, size and volume percentage of glass fiber and porosity defect inside glass fiber RRPUF was analyzed by CT analysis software. It shows that microfocus cone-beam X-ray industrial computed tomography is important method in non-destructive testing for RRPUF structure characteristic.