Abstract: A high-speed and multi-channel data acquisition system with PGA of the medical is proposed. Using FPGA, serial register, and analog multiplexer to control the input signals to ADC4344 A/D converter. Using PGA (Program Gain Amplifier) to improve the system resolution. The system circuit is simple which is universal to with high-speed, multi-channel, wide dynamic range data acquisition systems, and can be extended according to the system.