Abstract: objective: To study the value of multi – helical(slice) CT with 3D reconstruction in the observation and measurement of original shape of crop(plant) root system. Methods: Nine flowerpots of three kinds of crop which were growing in three different culture mediums were scanned with multi-slice CT(MSCT), The volume parameters of the root system of the 3 plants were transferred to the CT 3D workstation. Soft wares including MIP,SSP,MPR and VR for the processing of the figures were employed to construct the images. The images were evaluated by 2 physicians specialized in CT and 2 agriculture specialists. Results: The attempt to get in situ plant root images was made with using MSCT imaging technology and implemented visualization of plant root growing in different medium. Conclusion: The root system of plants cultured with organic media can be shown with MSCT,and MIP is the most effective software to display the root system.