Abstract: Objective: To evaluate the application value of dual-source computed tomography (CT) three-dimensional (3D) stereo localization combined with low-dose scanning in lung biopsy to improve the success rate of puncture and reduce radiation dose. Methods: Sixty patients who underwent CT-guided lung biopsy in our hospital between June 2024 and October 2024 were selected and randomly divided into control and experimental groups (30/group). The control group underwent conventional CT positioning combined with routine-dose scanning, whereas the experimental group underwent dual-source 3D stereotactic CT positioning combined with low-dose scanning. The puncture success rate, operation time, radiation dose, and complication rates were compared between groups. Results: The experimental group had 29 successful punctures (29/30), and the control group had 27 successful cases (27/30). The success rate of the experimental group was slightly higher than that of the control group, but the difference was not statistically significant. The operation time in the experimental group was (20.6±0.596) min, significantly shorter than 30.33±0.687 min in the control group, with a statistically significant difference. The experimental group had three complications (3/30), while the control group had seven (7/30). The complication rate in the experimental group was slightly lower than that in the control group, but the difference was not statistically significant. The volume CT dose index (CTDI_vol), dose-length product (DLP), and average effective dose (ED) in the experimental group were (2.443±0.055) mGy, (18.653±0.686) mGy·cm, and (0.317±0.012) mSv, respectively. In the control group, these values were (4.958±0.031) mGy, (42.580±0.544) mGy·cm, and (0.724±0.009) mSv, respectively. The radiation dose in the experimental group was significantly lower than that in the control group. Conclusion: Dual-source CT 3D stereo location combined with low-dose scanning provides significant advantages for lung biopsies. This can significantly improve the accuracy of puncture positioning, reduce the occurrence of complications, reduce the radiation dose, and improve the puncture success rate. This technique offers a safer and more effective approach for the diagnosis and treatment of lung diseases and may be considered for broader implementation in clinical practice.