Abstract: Multiple endocrine neoplasia (MEN) refers to the occurrence of tumors in two or more endocrine glands, with low incidence, variable symptoms, and difficult diagnosis. This paper reports a case of MEN1-like syndrome with a negative genetic test. Based on laboratory test results, imaging, and pathological results, the patient was considered to have MEN1, and the whole-exon sequencing (including mitochondria) was negative, so it was diagnosed as MEN1-like syndrome. Pathological immunohistochemistry confirmed a pituitary adenoma, and a parathyroid adenoma was revealed by parathyroid pathology. Subsequent examinations revealed an adrenal adenoma and a mammary adenosis nodule.