Abstract: Objective: To explore the diagnostic value of CT combined with serum CA125 and HE4 in ovarian epithelial malignant tumor. Methods: 156 cases of epithelial ovarian tumors (benign 72 cases, malignant 84 cases) were studied. Detections of CT and serum tumor markers CA125 and HE4 were carried out before operation, the diagnostic results were compared with pathology, for the evaluation of the diagnostic value of CT, CA125, HE4 and combined diagnosis in epithelial ovarian tumors. Results: The positive rates of serum CA125 and HE4 of malignant tumor group were higher than those of benign tumor group; The diagnostic sensitivity of CA125 for malignant tumor was higher than HE4, and the specificity was lower than HE4; the diagnostic accuracy of CA125 combined with HE4 for malignant tumor was higher than CT; the sensitivity, specificity and accuracy of CT combined with serum CA125 and HE4 for malignant tumor was 95.2%, 88.9% and 92.3% respectively, and were higher than CT or tumor markers alone. Conclusion: CT combined with serum CA125 and HE4 shows important value in the diagnosis of ovarian epithelial malignant tumor, which is conducive to early diagnosis and precise staging, and is worthy of promoting in application.