Abstract: Objective: To explore the application value of multi-detector CT and MRI in the diagnosis of middle-aged and senior females with hyperostosis frontalis interna (HFI). Methods: We performed retrospective analysis on the multi-detector CT and MRI images of the head of 102 middle-aged and senior females whose age ranged from 45 years old to 97 years old with the average age of 67.66±11.95 years old. VR post-processing images were used to evaluate the types of HFI while the thickness of the frontal internal plate was measured by CT, MRI sagittal and CT oblique sagittal images. We not only analyzed the difference between the thickness of the inner frontal plate measured respectively by CT and MR, but also explored the relationship among the age, thickness of the inner frontal plate and types of hyperostosis frontalis interna. Results: There were 29 cases in the middle-aged group whose age ranged from 45 years old to 59 years old with the average age of 52.97±4.43 years old and there were 73 cases in the senior group whose age ranged from 60 years old to 97 years old with the average age of 73.49±8.45 years old. The prevalence of HFI was respectively 41.38% in the middle-aged group and 56.16% in the senior group. There was no statistical difference between the two groups. In the HFI group, the prevalence of severe HFI was respectively 41.67% in the middle-aged group and 56.10% in the senior group. There was no statistical difference between the two groups. There was no statistical difference between CT sagittal and CT oblique sagittal measurements of frontal internal plate thickness, and there was no statistical difference between CT sagittal and MR sagittal measurements of frontal internal plate thickness. There was no significant difference in the thickness of the inner frontal plate between the middle-aged group and the senior group. The thickness of the inner frontal plate in the HFI group was greater than that in the non-HFI group. Regression analysis showed that there was no linear relationship between age and frontal plate thickness in middle-aged and senior females. Conclusion: The multi-detector CT and MRI can visualize and measure the thickness of the frontal bone internal plate, and VR post-processing images can visually reflect the internal surface of the frontal bone, which is beneficial for the diagnosis and classification of hyperostosis frontalis interna.