Abstract: Objective:To explore the application value of PET/CT image assisted CT guided lung biopsy. Method:Retrospective collection of 53 cases of pulmonary puncture under the guidance of PET/CT image assisted by CT in our hospital and 120 cases of lung puncture under conventional CT guidance. Count the basic clinical data(age, gender, emphysema), lesion status(size, location), puncture status(puncture position, depth, satisfaction with material sampling, number of times of sampling), and complication status(bleeding, pneumothorax, implantation, air embolism) and pathological results, statistical analysis. The test level is P < 0.05 with statistical significance. Results:There were statistically significant differences between the two groups of patients with satisfactory results in one material extraction, one puncture and two punctures, P < 0.05. The positive rate of pathological results is statistically different, P < 0.05. There was no statistical difference in clinical and puncture related data and total complications, P > 0.05. Conclusion:Compared with conventional CT-guided lung puncture, PET/CT assisted lung puncture has a higher positive rate of pathological results, and can significantly reduce the number of secondary chest biopsies and reduce the occurrence of complications.