Abstract: Objective:To evaluate the diagnostic value of CT-guided percutaneous needle biopsy in benign and malignant Solitary pulmonary nodule, and to analyze the factors affecting the diagnostic accuracy. Method:Forty patients were randomly divided into two groups:Study Group (CT-guided percutaneous lung biopsy with coaxial needle) and Control Group (CT-guided percutaneous lung biopsy with Papanicolaou needle). 40 patients with pulmonary Solitary pulmonary nodule were found in our hospital from September 2017 to July 2019, each group of 20 cases, according to CT scan image to choose the best puncture layer and puncture point to obtain specimens. The success rate, diagnostic accuracy and complications of puncture biopsy were compared between the two groups. Results:The success rate and diagnostic accuracy of puncture biopsy in the study group were higher than those in the control group (P<0.05), and the diagnostic sensitivity and specificity of malignant SPN were 92.86% and 90.00%, respectively, which were significantly higher than those in the control group. The results of CT-guided percutaneous biopsy were consistent with those of operation or follow-up (Kappa=0.725, P=0.000). The size of puncture focus is a factor that affects the diagnosis of SPN by CT-guided needle biopsy. Conclusion:CT-guided percutaneous needle biopsy with coaxial location needle can improve the success rate and diagnostic accuracy, reduce the risk of puncture-related complications, and has a high consistency between diagnosis and pathological results of SPN.