Abstract: Objective: To compare the Accuracy and stability of liver volume measurements by MDCT and MR by IQQA-liver auto analysis software. Method: (1) 12 rabbits were scanned by MDCT and MR FIESTA and LAVA sequences, Then kill the rabbits and measure the rabbits, liver immediately (actual liver volume ALV) by means of water displacement. Compare the liver volumes measured by MDCT, MR and water displacement. (2) Repetitive study: repeat measure the liver volumes of all the rabbits. Results: (1) The liver volumes of rabbit measured by MDCT, MR FIESTA and LAVA sequences were (126.4±35.8) cm³, (125.0±35.4) cm³, (125.8±35.7) cm³, had a significant correlation (r=0.997, 0.997, 0.998, P<0.05) with the liver volume by means of water displacement which was (126.6±36.4) cm³. Liver volume with MDCT, MR FIESTA and LAVA sequences and displacement of water was good consistency by Bland-Altman method. (2) Repetitive study: repeated measures of liver volumes of rabbits and patients at different time by each same operator, the ICC were 0.995, 0.985; measures of liver volumes of rabbits by two operators at each time, the ICC were 0.996, 0.987. Conclusion: MDCT and MR can provide accurate information in the measurement of the liver volume used by IQQA-Liver auto analysis software, with high reliability and reproducibility.