摘要:
目的:应用野百合碱(MCT)诱导建立大鼠肺动脉高压(PAH)模型,运用微型CT和磁共振心功能成像方法,研究肺动脉压力演变和右心室结构、功能演变之间的关系。材料与方法:将MCT诱导的PAH模型组(1 wk-PAH组、2 wk-PAH组、3 wk-PAH组、4 wk-PAH组)和每一个模型组的对照组大鼠分别随机分组(每小组12只),分别在第1、2、3和4周右心导管测量肺动脉压力,微型CT测量主肺动脉直径及右心室与左心室最大径比值,MRI右心功能动态检测,观察肺动脉压力和MRI参数演变关系。比较对照组、PAH模型组各组间的相关各参数差异。采用SPSS 17.0统计软件,应用Pearson相关性分析,评价右心室射血分数,右室舒张末期容积,右室收缩末期容积,右心室与左心室最大径比值和主肺动脉直径分别与平均肺动脉压的相关性,组间比较采用完全随机分组的t检验,P<0.05为差异具有统计学意义。结果:注射野百合碱后1至4周,48只模型组大鼠的右心室射血分数、右心室舒张及收缩末期容积与平均肺动脉压有很好的相关性(分别为rRVEF=-0.823,rRVEDV=0.732,rRVESV=0.803)。右心室与左心室最大径比值与平均肺动脉压有轻度相关(r=0.694),主肺动脉直径与平均肺动脉压无相关性,r=0.438。注射野百合碱前两周,野百合碱组大鼠的平均肺动脉压、射血分数、右室舒张末期和收缩末期容积、右心室与左心室最大径比值与对照组比较未见显著差异(P>0.05)。3周到4周后,以上各参数与对照组比较有显著性差异(P<0.05)。结论:随着大鼠的肺动脉压增高,右心室与左心室最大径比值增加,右心室射血分数逐渐降低,右心室舒张末期及收缩末期容积逐渐增加。对于大鼠慢性肺动脉高压模型的监测,CT和MRI可以准确快速测量各项参数变化,简单易行。右心室舒张末及收缩末期容积、射血分数等参数是提示肺动脉高压的敏感参数。
Abstract:
Objective: The aim of our study was to establish the rat models of pulmonary hypertension induced by monocrotaline. Using the micro CT and magnetic resonance (MR) right ventricular function imaging methods, we research the relationship between the evolution of right ventricular structure and function and the evolution of pulmonary artery pressure (PAH). Methods: Ninety-six Sprague-Dawley rats were randomly assigned into two groups: control group (n=48), and four PAH groups (n=48) caused by monocrotaline (MCT). Each group were divided into four groups (1 wk-PAH group, 2 wk-PAH group, 3 wk-PAH group, 4 wk-PAH group). We measured pulmonary artery pressure by right heart catheterization. After injection of MCT, we used the micro CT and MRI to measure the pulmonary artery diameter and the right ventricular function of the rats every week. All measurements of RV structure and function in model group were compared with pulmonary average pressure (PAP) using Pearson's correlation. Results: There were strong correlations between the parameters of RV structure and function in model group with the pulmonary average pressure (r=-0.823 for RV EF, r=0.732 and 0.803 for RV EDV and RV ESV, r=0.694 for the maximum inner diameter ratio of right ventricle to left ventricle). The diameter of main pulmonary artery was not correlated with mean pulmonary artery pressure (r=0.438). After injecting monocrotaline two weeks, the PAP, the maximum inner diameter ratio of right ventricle to left ventricle,, right ventricular eject fraction (RVEF), end-diastolic volume (EDV) and end-systolic volume (ESV) of right ventricle between rats in PAH and the control group showed no significant difference (P > 0.05). But three-four weeks after MCT injection, all these parameters were significant differences in PAH rats than in control group (P < 0.05). Conclusions: As the pulmonary arterial pressure increased in rats, the right ventricular function was gradually impaired. For the monitoring of chronic pulmonary hypertension model in rats, CT and MRI can accurately and rapidly measure the changes of parameters. The PAH can be indicated by observing parameter changes such as the pulmonary artery diameter, the right ventricular volume and cardiac output.
百度学术
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