Abstract: Objective: To investigate the multi-slice CT reconstruction techniques in the diagnosis of pulmonary sequestration value to evaluate it for the role of the clinical diagnosis of pulmonary sequestration. Method: By surgery and pathology confirmed 13 cases of pulmonary sequestration were retrospectively analyzed. All checks with GE lightspeed 64-slice spiral CT Scanner, the first CT scan, the line dual-phase enhanced scan, the scan line after the end of the multi-planar reconstruction(MPR), maximum intensity projection(MIP) and volume rendering image(VR). Results: 13 cases of pulmonary sequestration patients, 10 cases of lesions located in the left lower lobe basal segment, 3 cases of lesions located in the lower lobe basal segment, after the abnormal body from 9 cases of thoracic aortic artery, 4 cases of abdominal aorta. CT scan of leaf shape(3 cases), circular or ovoid in 3,7 cases of irregular shape. Enhancement and reconstruction after 13 cases showed abnormal blood flow in arteries. Conclusion: Multislice CT and reconstruction techniques can accurately display abnormal arterial blood supply and venous return, in the isolated pulmonary sequestration has significant value in diagnosis and preoperative assessment.