Abstract: Objective: To explore the clinic value of Multi-slice spiral CT-virtual hysterosalpingography in diagnosing fallopain tube patency. Methods: The pelvic cavity MSCT was performed on 40 infertility patients, MSCT-VHSG was performed with a 64-row CT scanner using 64×0.625 mm² collimation and 0.625 mm slice thickness. A total volume of 20 mL of an iodine contrast dilution was injected into the uterine cavity. The duraion of the CT scan and the grade of patient discomfort of the procedure were documented. Images were analyzed on a workstation. Retrospective thin-slice reconstruction 0.625 mm to 1.5 mm after multi-slice spiral CT scan, the techniques such as MPR, CPR, VR and MIP, were used to observe fallopain tube patency. Results: No complications occurred during the procedure. The mean scan time was(3.4 ±0.4)s; the mean patient effective dose was(2.58 ±0.75)mSv. The stereo pictures of the uterus cave were obtained on MSCT-VHSG. The fallopian tube was clearly visualized without obvious adverse reactions occurring. Forty fallopian tube were diagnosed as patent,8 as partially patent and 32 as non-patent by MSCT-VHSG. Conclusion: A 64-row CT-VHSG provides a reliable, non-invasive alternative diagnostic technique in the infertility workup algorithm.