Abstract: Objective: To investigate the value of multislice spiral computed tomography(MSCT) in the diagnosis and differential diagnosis of primary gastrointestinal lymphoma. Methods:The clinical imaging data of 40 cases of primary gastrointestinal lymphoma confirmed by surgery and pathology in our hospital were retrospectively analyzed. Results:40 PGILs all belonged to non-Hodgkin's lymphoma, including 31 cases of primary gastric lymphoma(PGL) and 9 cases of primary small intestinal lymphoma(PSIL). 19 cases of PGL showed diffuse homogeneous gastric wall thickening in MSCT. They all clearly showed mucosa white line sign and track sign. The wall thickening of the gastrointestinal tracts were not consistent with the stenosis of the lumens of the lesions occurred in 39 cases. 4 cases showed aneurysm-like intestinal dilatation. 1 case showed the hamburger sign. Clinical imaging rarely showed obstruction. Conclusion:To recognize the typical CT imaging features of primary gastrointestinal lymphoma, can improve the preoperative diagnostic accuracy of the disease.